Fig. 8
From: Accuracy of the detection of binding events using 3D single particle tracking
Limits to the detection of changes in diffusion. D(t) plots obtained from the rolling-window analysis (a, c, e) and scatter plots of D gap vs the length of the gap (b, d, f). The simulated D(t) is plotted in black line. The D outside the gap was set to 0,05 μm2/s. D gap = 0.0001 μm2/s for a, b, D gap = 0,01 μm2/s for c, d and D gap =0,035μm2/s for e, f. For every case, 100 traces were simulated and analyzed: a, c and e show the D(t) plot for 8 example traces. Results from all 100 traces are shown in b, d and f. The ranges of correct D gap and t gap are highlighted with blue lines in the scatter plot. The traces were analyzed using a rolling window and a Welch sample of 15 points