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Figure 6 | BMC Biophysics

Figure 6

From: Molecular dynamics and mutational analysis of the catalytic and translocation cycle of RNA polymerase

Figure 6

Exonuclease III mapping of Sc RNAP II TECs. A) Back border (upstream border) 3′-dA11 Sc RNAP II TEC mapping. 3′-dA11 (3′-dATP chain terminated A11 TECs) RNA controls, run in the same reaction, are shown to demonstrate effective chain termination at the A11 position. The strategy for mapping Sc RNAP II TECs in the absence or presence of incoming CTP is indicated at the bottom of the figure. B) Phosphorimager quantification of triplicate determinations done at 30 s exonuclease III incubation. C) Percent increase in the post-translocated state induced with CTP compared to wild type Sc RNAP II, from the data shown in B. Error bars represent standard deviation.

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