Figure 8
NMR hydrogen-deuterium (H/D) exchange. (a) Superimposition of the 1H-15N NMR HSQC spectra of the 15N-labeled EphA4 LBD at 25°C in the buffer (blue) and 15 min (red) after the lyophilized EphA4 LBD powder was re-dissolved in D2O. (b) Superimposition of the 1H-15N NMR HSQC spectra of the 15N-labeled EphA4 LBD at 25°C, 15 min (blue) and 2 hr (red) after the lyophilized EphA4 LBD powder was re-dissolved in D2O. (c) The structure of the EphA4 LBD with the H/D exchange results mapped onto. Blue: the residues completely exchanged within 15 min; green: residues completely exchanged from 15 min to 2 hr; red: residues un-exchanged after 2 hr.