Time-lapse series of FA dynamics in MTLn3 cells. MTLn3 cell expressing paxillin-EGFP and stimulated with 0.01 nM EGF is shown. Images were taken at 0, 10, 20, 30 and 40 min. A. Original total internal reflection fluorescence (TIRF) images of cells with FAs. B. Binary images of whole cell masks after segmentation. C. Binary images of FA masks after segmentation. D. Composite images of tracked FAs within the cell, where green represents original images and red represents the segmented FA masks. The scale bar is 10 μm.