Figure 6

The spatial control of protrusion differs between slow and fast migrating cells. A. Protrusion velocity map for slow migrating cells at 0.01, 1, and 100 nM EGF from left to right. B. Protrusion velocity map for fast migrating cells at 0.01, 1, and 100 nM EGF from left to right. The cell edge was divided into 100 segments and the average protrusion rate in each segment was determined over time. Red represents fast protrusion, green represents quiescence and blue represents fast retraction. C. The Kolmogorov-Smirnov statistic was the average of three pair-wise comparisons (EGF) or simply the pair-wise comparison (cell speed). A larger value for the Kolmogorov-Smirnov statistic signifies a higher probability that there are differences between groups. D. The fraction of cells with lateral waves (gray bars) or high standard deviation (STD) of protrusion velocity (white bars) between slow and fast migrating cells. E. Histograms of protrusion/retraction velocity between slow migrating cells (gray bars) and fast migrating cells (white bars). Histograms of slow (dot lines) and fast migrating cells (solid lines) were fit with Gaussian distribution. F. The mean values of standard deviation (STD) of protrusion velocity between slow and fast migrating cells were also shown. N _slow  = 34, N _fast  = 24. Error bars are 95 % confidence intervals and asterisks denote p < 0.01.