Influence of NO on denaturation onset of Hb samples. Influence of NO on denaturation onset of Hb samples (3 in each group) prepared in Na buffer (a) and the K buffer (b). Arrows indicate the appearance of an additional peak in the NO-treated samples that generally denatured significantly earlier than the control samples. In the region between 25°C to 45°C the Rh values for hemoglobin were around 5 nm in the Na buffer and around 4 nm in the K buffer, depending on the sample. For the temperatures exceeding 65°C the exact Rh values could not be measured because of pronounced size heterogeneity of the formed aggregates.