Figure 1
From: Effects of spermine NONOate and ATP on protein aggregation: light scattering evidences
Influence of NO on denaturation onset of Hb samples. Influence of NO on denaturation onset of Hb samples (3 in each group) prepared in Na buffer (a) and the K buffer (b). Arrows indicate the appearance of an additional peak in the NO-treated samples that generally denatured significantly earlier than the control samples. In the region between 25°C to 45°C the Rh values for hemoglobin were around 5 nm in the Na buffer and around 4 nm in the K buffer, depending on the sample. For the temperatures exceeding 65°C the exact Rh values could not be measured because of pronounced size heterogeneity of the formed aggregates.