Figure 3
From: Improving accuracy of cell and chromophore concentration measurements using optical density
Depiction of spectrophotometer differences for absorbing and scattering samples. A) The ratio of the OD of different dyes measured on two spectrophotometers (the Molecular Devices Spectra Max 384 over the Beckman DU520) is plotted against wavelength to demonstrate the variability of spectrophotometers that is observed even for simple absorbing dye samples. An E. coli culture is included in the figure to illustrate how a predominantly scattering sample impacts the observed difference between these spectrophotometers. B) Absorption spectra of the dyes used.