Figure 9
From: Improving accuracy of cell and chromophore concentration measurements using optical density
Online implementation of the OD flow cell to monitor an E. coli fermentation. Measurement of OD time course in a 5-L fed-batch E. coli fermentation using a peristaltic pump to pass culture through the flow cell in a recirculation loop. The OD flow cell was implemented with a 595 nm LED (Ligitek, LHY3833, 2700-mcd, 12o). The true optical density was measured offline after dilution to the non-saturating biomass concentrations on Beckman spectrophotometer. The inset is a plot of the non-diluted OD-biomass correlation measured in the flow cell as compared to the offline spectrophotometer.