Figure 5

Oligonucleotide immobilization in liquid solution. Mean fluorescence intensities of liquid phase immobilization of aACG and ACG at concentrations of 10 and 50 μM, respectively, in 150 mM phosphate buffer after 20 hrs on 2D epoxy and on 3D NHS hydrogel slides. Measurements were performed with the same scanner settings as applied in the nL experiments to allow comparing intensities.