Effect of deuteration on Raman spectra of ASBVds. The Raman spectra of minus (panel A) and plus (panel B) strands of ASBVd are obtained in cacodylate buffer, in the absence of Mg2+. Blue curve (a) correspond to Raman spectra in H2O; red curve (b) correspond to Raman spectra in D2O. All spectra were normalized using the Raman band at 1100 cm-1; the contributions from the cacodylate buffer and quartz cell were subtracted. The difference spectrum (c) (c = b-a, black curve) correspond to spectral changes due to H to D atom substitution.