Figure 5

Effect of Mg²⁺binding on Raman spectra of ASBVds. The Raman spectra of the minus (panel A) and plus (panel B) strands of ASBVd are obtained in cacodylate buffer. Blue curve (a) correspond to Raman spectrum in the absence of Mg²⁺; red curve (b) correspond to Raman spectrum in the presence of 20 mM Mg²⁺. Spectra were not normalized in order to see the effect of Mg²⁺ on the phosphodioxy band at 1100 cm^-1, but the contributions from the cacodylate buffer and quartz cell were subtracted. The difference spectrum (c) (c = b-a, black curve) was multiplied by a factor of 3 to visually enhance the resulting spectral changes corresponding to Mg²⁺ binding at 20°C.