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Table 1 Biophysical property of Treg cells by detection of AFM

From: AFM detection of biophysical characteristics of specific regulatory T Cells after allogeneic hematopoietic stem cell transplantation

Groups

Width (W) (μm)

Peak (H) (nm)

Ra (nm)

Total CD4 + CD25+ cells

12.04 ± 0.24

3.95 ± 0.27

250.83 ± 82.39

Total CD4 + CD25- cells

10.23 ± 1.54

3.27 ± 0.32

110.45 ± 22.11

P values*

< 0.001

< 0.001

< 0.001

CD4 + CD25+ with GVHD

11.96 ± 0.17

3.85 ± 0.14

191.33 ± 75.22

CD4 + CD25+ without GVHD

12.12 ± 0.13

4.12 ± 0.21

311.67 ± 18.45

P values*

0.032

< 0.001

< 0.001

  1. Ra: average roughness Ra= 1 / N ∑ n = 1 N Z n − Z ¯ ; Z ¯ : given by the average height within the included areas defined by a region group Z ¯ = 1 / N ∑ n = 1 N Z n ; The Z is the height profile line based on the surface.
  2. *Compared by the Welch test.