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Figure 1 | BMC Biophysics

Figure 1

From: Mathematical modelling of the automated FADU assay for the quantification of DNA strand breaks and their repair in human peripheral mononuclear blood cells

Figure 1

Signal fluorescence depending on irradiation dose. Human peripheral blood mononuclear cells (PBMC) were irradiated with several doses of x-ray. DNA strand breaks formation was measured using the automated FADU assay exactly as described [1].The Sybr-green fluorescence intensity, which is a direct marker of double-stranded DNA, decreases with increasing the irradiation dose, due to progressive alkaline unwinding of the DNA in the lysate starting from DNA ends or breaks. Circles represent the mean of three experimental replicates. Each graph represents the one donor.

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BMC Biophysics

ISSN: 2046-1682

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