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Figure 2 | BMC Biophysics

Figure 2

From: A novel delta current method for transport stoichiometry estimation

Figure 2

HCO 3 -induced current in NBCe2-C expressing HEK-293 cells. a) The cell was whole-cell voltage-clamped at -60 mV. A series of 400 ms voltage-clamp pulses range from -95 to +45 mV with increment of 10 mV were applied and whole-cell current responses were recorded. In the pre-HCO3 conditions, there is no HCO3 in the patch pipette (Table 1, patch solution a) nor in the bath solution (Table 1, Bath solution A). Increasing HCO3 concentration to 25 mM in the bath solution (Bath solution B in Table 1) induced a voltage-dependent current (central panel). The current recovered when the cell was washed with solution containing 0 HCO3 (right panel). b) Current-voltage (I-V) relation of steady-state current in the absence and presence of HCO3 (n = 8). Im (pA): membrane current in pA. Steady-state current was obtained by averaging 80 ms of the current trace toward the end of each 400 ms voltage pulse. c) I-V curve of HCO3 induced current is the difference between the I-V curves in the absence of HCO3 and in the presence of HCO3 . d) Application of 25 mM HCO3 in the bath did not induce any current in EGFP negative cells (n = 4).

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