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Table 1 Solutions

From: A novel delta current method for transport stoichiometry estimation

Components

Pipette

Bath

a

b

c

d

A

B

C

D

E

NaCl

    

110

110

55

 

15

CsCl

    

10

10

10

  

CaCl2

1

1

1

1

1.5

1.5

1.5

1.5

1.5

MgCl2

    

1

1

1

1

1

TEA-Cl

10

10

10

10

     

TMA-Cl

      

55

120

105

EGTA

10

10

10

10

     

HEPES

10

50

50

50

10

10

10

10

10

NaHCO3

 

8

25

25

 

25

25

10

10

Cs-Gluconate

125

105

105

90

     

Cs-HCO3

 

17

       

TMA-HCO3

       

15

15

Na-Gluconate

10

  

15

25

    

ATP-Mg

1

1

1

1

     

ATP-Na2

1

1

       

Glucose

    

15

15

15

15

15

  1. Bicarbonate-containing solutions were bubbled with 5% CO2 and 95% O2. All solutions were pH 7.4. Glucose was included in the bath solutions to adjust the osmolality to approximately 300 mmol/Kg. The solution osmolality was determined with an osmometer (Model 5520, Vapro® vapor pressure osmometer, Wescor Inc., Logan, UT, USA).
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BMC Biophysics

ISSN: 2046-1682

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