Figure 2
From: A nucleotide-independent cyclic nitroxide label for monitoring segmental motions in nucleic acids
Characterization of R5c-labeled oligonucleotide duplexes. (A) cw-EPR spectra of \( {S}_c^{R5c} \) (top, cyclic nitroxide attachment) and \( {S}_s^{R5c} \) (bottom, linear nitroxide attachment). RNA duplexes were formed by pairing with the complementary IGS strand (see Table 1). (B) Thermal denaturation results. Errors in the reported ΔG0 37°C values were estimated to be < 1.0 kcal/mol from repeated measurements.