Figure 3
From: A nucleotide-independent cyclic nitroxide label for monitoring segmental motions in nucleic acids
Monitoring P1 motions in group I ribozymes. (A) Schematic representation of the ribozyme open (top) and closed (bottom) complexes. The substrate is represented by the red line, and the cleavage site is indicated by the red triangle. R5c labeling site is marked by the yellow dot. (B) EPR spectra of the R5c-labeled P1 duplex in isolation and as part of the wild-type ribozyme open complex. (C) Spectral comparisons between wild-type (3A) and mutant (3U) ribozymes containing R5c-labeled P1. Amplitude of the difference spectra (green) was scaled by 2-fold. (D) Spectra of R5c-labeled P1 in the wild-type ribozyme open and closed complexes.