Figure 4

Influence of the linker length. Changes in TCSPC histograms were investigated as a function of different lengths of linker between dyes and labeling positions. These differences were based on equal amounts of FRET pairs with either 3 nm or 6 nm distance between the labeling positions (A) or distance positions of a polyglutamine fibril model (B) as described in [20]. Note that the time scales are different in order to better resolve an important area of signals before reaching the background.