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Fig. 5 | BMC Biophysics

Fig. 5

From: Copper-free click chemistry for attachment of biomolecules in magnetic tweezers

Fig. 5

Anchored DNA molecules can be torsionally constrained and withstand forces of >100pN. a The DNA molecules anchored with click chemistry show the expected behavior (a strongly rising force, and for unconstrained molecules, a plateau near 65pN as DNA overstretches and a further rise) in a slow force ramp of 1pN/sec. Different colors represent different tethers. All tethers that were bonded by click chemistry withstand forces of over 100pN. By contrast, the DNA anchored with digoxygenin/anti-dig (black) breaks off near 40pN, well before the overstretching point. b Rotation curves at constant forces of (light to dark) 0.5, 1, 3 and 5pN, indicating that this 20 kb DNA molecule anchored with click chemistry is torsionally constrained

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