Fig. 3

Intensities changes of amide groups in αS at various concentration of Tb³⁺ or Dy³⁺. The I/I₀ profiles of α-synuclein backbone amide groups were plotted as a function of residue number at molar ratios of (a) αS/Tb³⁺(4/1), (b) αS/ Tb³⁺(2/1), (c) αS/Tb³⁺(1/1), (d) αS/Dy³⁺(4/1), (e) αS/Dy³⁺(2/1), (f) αS/Dy³⁺ (1/1), respectively. The residues that were severely broadened were labelled in red rectangle. According to the attenuation degree of cross peaks (I/I₀), the whole residues of αS were divided into three parts: residues 1 to 97, residues 98 to 109, and residues 110 to 140, which were all labelled with orange lines. αS sequence shown above the figure is the same as that in Fig. 2