Fig. 3

Outputs of quick analysis. Results of simple case analysis of good quality video. a Frame from the video, t=5.0 s, contrast is sufficiently high and stable. The bead is marked by the red ring, the point tracked on the pipette in blue, the pipette tip pattern outlined by the white dashed box. b Tracking quality metrics of the bead (red) and correlation coefficient of the pipette (blue). Note that the bead detection quality is very volatile as compared to the pipette. c Tracks of the bead centre (red) and the pipette anchor point (blue) during the experiment time course. In the inset, part of the bead track is magnified; the last second of the path, delimited by the time points 6 s and 7 s, is highlighted (rest is faded). The time point 6.6 s denotes the probe rupture—see panel D. Fluctuations of the bead along the Y-coordinate (perpendicular to the pipette movements) are of order 10 nm. d Deformation of the RBC (Δ x) and the force exerted by the probe during the experiment. Red dashed line indicates the zero force, the red mark indicates a separation of the RBC and bead. Total computational CPU time 105 s. The video file used for this analysis, basic.avi, is included in the program repository