Fig. 1
From: Accuracy of the detection of binding events using 3D single particle tracking
Steps to extract the diffusion constant of single GNRs in glycerol. From a movie of 3D stacks of frames (as the frame in a), trajectories of single GNRs were extracted (b), and on each of them a Mean Square Displacement (MSD) analysis was performed. The MSD plot (c) was fitted to a line with a slope that corresponds to the diffusion coefficient, and offset proportional to the 3D positional accuracy