Fig. 2

Detection of diffusion transitions. a A simulated trajectory, which contains a brief period of reduced mobility. In this gap D was 0,0001 μm²/s, while the D in the rest of the trace was 0,05 μm²/s. The gap is highlighted with a green circle. b The diffusion coefficient along the trajectory D(t) was calculated using a 15 points rolling-window method. c A Welch test yielded minima in the P-value plot, that indicate possible transition points used to divide the trajectory in subtraces. d MSD analysis of each subtrace yielded D values as shown