Fig. 9
From: Accuracy of the detection of binding events using 3D single particle tracking
Limits to the detection of short-lived changes in diffusion. D(t) plots were obtained from rolling-window analysis (a, c) and the results of the MSD analysis are presented as scatter plots in c and d. The simulated D(t) is plotted in black line. The D gap was 0.01 μm2/s and the D outside the gap was 0,05 μm2/s. t gap = 25 s in a, b and t gap = 10 s in c, d. The results can be compared to Fig. 8 (c, d) where the same Ds were set with a gap length of 100 s. For every case 100 traces were simulated and analyzed: a and c show D(t) for 10 example traces; from all the traces are shown in b and d. The ranges of correct D gap and t gap are highlighted with blue lines in the scatter plot. The traces were analyzed using a rolling window size of 15 points, and a Welch sample of 15 points (b) and 10 points (d)