Fig. 3
From: GPMVs in variable physiological conditions: could they be used for therapy delivery?
LPS caused the formation of vesicle-like evaginations and tethers from the ld phase of GUVs. Shape change of a representative GUV in a microfluidic diffusion chamber under bright field (a-d) and the same vesicles under fluorescence. The ld phase was marked with NBD-PC marker. Initially, the osmolality of the surrounding buffer was changed from 300 to 330 mOsmoL/kg, making the vesicle more flaccid (a, e). After 8 min, 10 μg/mL LPS was added and tether-like evaginations developed (b, c, f, g). The arrows in images c and g point at a tether. When LPS was replaced with LPS-free solution, GUV occupied their original shape (d, h). The GUVs were imaged under an inverted microscope in bright-field and epi-fluorescence mode (magnification 60X). The bar represents 10 μm