Fig. 5

When LPS was replaced with LPS-free solution, GUV occupied their original shape as seen on the bright-field image (I) and no fluorescent signal could be detected (II). In GPMVs the signal from fluorescent LPS remained in the vesicles after LPS removal. GPMVs exposed to 10 μg/mL FITC conjugated LPS in the microfluidic diffusion chamber for 10 min. The surrounding LPS solution was then switched to GPMV buffer without LPS (a). The bound LPS signal was visible in some vesicles (arrows) even after the depletion of LPS from the solution (b). The vesicles were imaged with a confocal microscope (magnification 60X). The bar represents 10 μm