Fig. 5

(a-d) are the original FLIP images of the CHO cells expressing GFP-Q73 in the cytoplasm and nucleus. The black circle on the image a shows the 18-pixel wide bleaching area in the nucleus with a pixel size of 0.0624404 μm and a scalebar which is 5 μm. a is taken before bleaching, b is after 10 time bleaches, i.e., time t=26 s. c is the 20’th FLIP image in the sequence (time t=52 s) and d is produced at time t=104 s which correspond to FLIP fame 40. The second row e-h shows the corresponding goal function. The third row i-l shows the simulation results, all at times corresponding to the displayed FLIP images