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Table 1 Experimental properties

From: Brownian dynamics simulation of analytical ultracentrifugation experiments

Molecule

γ-cyclodextrin

Lysozyme

Polyoxyethylene

DNA T7

1 v ¯ ρ

0.333

0.298

0.170

0.450

10−3M (g/mol)

1.50

14.3

300

26 000

s (S)

0.53

1.89

2.30

31.8

107D (cm3/g)

27.0

10.8

1.10

0.066

ω (rpm)

55 000

40 000

40 000

10 000

t run (secs.)

40 000

40 000

40 000

60 000

10−3M calc (g/mol)

1.44 (-4)

13.9 (-3)

300 (0)

23 278 (-10)

s calc (S)

0.54 (+2)

1.84 (-3)

2.26 (-2)

31.4 (-1)

107D calc (cm3/g)

27.4 (+1)

10.8 (0)

1.08 (-2)

0.073 (+11)

  1. Molecules and the experimental properties used as input in the simulation of sedimentation velocity experiments, and properties recovered from the simulation profiles after the SEDFIT analysis. Numbers between parenthesis are the percent deviations of the recovered values from those used as input for the simulations.