Table 1 Conversion factors between OD, dry weight and cell number
From: Improving accuracy of cell and chromophore concentration measurements using optical density
Species* | ODrobust | spec | gDW/ODrobust | CFU/ODrobust(x10-8) |
|---|---|---|---|---|
Escherichia coli (DH5α) | 600 | A | 0.396 ± 0.011 | 7.94 ± 2.0 |
600 | B | 0.515 ± 0.014 | 10.3 ± 2.6 | |
Agrobacterium tumafaciens (EHA105) | 600 | A | 0.409 ± 0.004 | 13.66 ± 7.06 |
660 | A | 0.485 ± 0.004 | 16.19 ± 8.37 | |
Agrobacterium rhizogenes (15834) | 660 | A | 0.410 ± 0.011 | 7.89 ± 0.95 |
A | 0.509 ± 0.014 | 9.40 ± 1.13 | ||
Azotobacter vinelandii | 600 | A | 1.920 ± 0.205 | 4.50 ± 1.91 |
Bacillus thuringensis (ssp. israelensis)## | 600 | A | 0.335 ± 0.006 | 0.519 ± 0.183 |
660 | A | 0.370 ±0 .007 | 0.573 ± 0.202 | |
Clostridium phytofermentans (ISDgT) | 600 | A | 1.64 ± 0.05 | 6.47 ± 1.34 |
600 | B | 2.43 ± 0.07 | 9.58 ± 1.98 | |
Rhodobacter capsulatus (SB1003) | ||||
Heterotrophic (aerobic) | 660 | A | 0.626 ± 0.048 | 244 ± 104 |
660 | B | 0.652 ± 0.052 | 251 ± 199 | |
600 | A | 0.500 ± 0.036 | 223 ± 164 | |
600 | B | 0.548 ± 0.042 | 261 ± 193 | |
Photoheterotrophic (anaerobic) | 660 | A | 0.419 ± 0.031 | 13.9 ± 5.4 |
660 | B | 0.499 ± 0.061 | 14.8 ± 6.1 | |
Autotrophic (H2, O2, CO2) | 660 | A | 0.563 ± 0.003 | 43.2 ± 5.6 |
660 | B | 0.602 ± 0.003 | 45.5 ± 5.9 | |
Rhodobacter sphaeroides (2.4.1) | ||||
Heterotrophic (aerobic) | 660 | A | 0.453 ± 0.144 | 367 ± 122 |
660 | B | 0.515 ± 0.128 | 378 ± 126 | |
600 | A | 0.466 ± 0.074 | 341 ± 113 | |
600 | B | 0.491 ± 0.111 | 312 ± 104 | |
Photoheterotrophic (anaerobic) | 660 | A | 0.553 ± 0.004 | 159 ± 27 |
660 | B | 0.606 ± 0.005 | 174 ± 29 | |
600 | A | 0.446 ± 0.004 | 128 ± 22 | |
600 | B | 0.548 ± 0.004 | 158 ± 27 | |
Ralstonia eutropha (h16) | ||||
Heterotrophic (aerobic) | 600 | A | 0.464 ± 0.017 | 11.5 ± 2.2 |
600 | B | 0.604 ± 0.022 | 13.3 ± 2.5 | |
Autotrophic (H2, O2, CO2) | 600 | A | 0.298 ± 0.007 | 16.7 ± 1.1 |
600 | B | 0.383 ± 0.009 | 20.8 ± 1.4 | |
Saccharomyces cerevisiae (Y‒562) | 600 | A | 0.644 ± 0.025 | 0.330 ± 0.083 |
600 | B | 0.848 ± 0.033 | 0.430 ± 0.109 | |
Candida molishiana (yeast) | 600 | A | 0.436 ± 0.011 | 0.498 ± 0.104 |
Synechocystis sp. PCC 6803 | 550 | B | 0.63 ± 0.11 | 0.380 ± 0.046 |
730 | B | 0.60 ± 0.10 | 0.478 ± 0.058 | |
Synechococcus OH2 | 750 | A | 0.361 ± 0.010 | -na- |
Synechococcus sp. (Marine ) | 750 | A | 0.350 ± 0.020 | 1.05 ± 0.100 |
Chlamydomonas reinhardtii (1690) | 550 | B | 0.530± 0.100 | 0.240 ± 0.028 |
Chlorella vulgarus | 550 | A | 0.316 ± 0.007 | 0.161 ± 0.019 |
550 | B | 0.430 ± 0.024 | 0.692 ± 0.077 | |
Botryoccoccus braunii (race B) | 550 | B | 2.15 ± 3 | -na- |