Table 2 Computational simulation of ΔI and ΔE rev methods to estimate q in the absence or presence of a Cl − channel
From: A novel delta current method for transport stoichiometry estimation
[HCO 3 −] i = [HCO 3 −] o = 25 | V I=0(mV) | ΔE rev(mV) | q (ΔE rev) | ΔI 2/ ΔI 0(V 2 = 12 mV) | q (ΔI) | |
|---|---|---|---|---|---|---|
[Na +] i=10 mM | [Na +] o = 10 | [Na +] o = 25 | ||||
q = 2 | 0 | −23.5 | −23.5 | 2.0 | 1.263 | 2.0 |
q = 3 | 0 | −11.75 | −11.75 | 3.0 | 1.595 | 3.0 |
q = 2, + GCl | −5.1 | −25.3 | −20.2 | 2.17 | 1.263 | 2.0 |
q = 3, + GCl | −12.8 | −18.75 | −5.95 | 4.96 | 1.595 | 3.0 |
q = 2, + 2 x GCl | −9.2 | −26.9 | −17.7 | 2.33 | 1.263 | 2.0 |
q = 3, +2 x GCl | −19.7 | −23.5 | −3.8 | 7.2 | 1.595 | 3.0 |