Fig. 6

Optimal number of MSD points. Simulations and experimental data show that two points of the MSD plot are optimal for accurate fitting of D. The squares represent experimental values of ρ, calculated using Eq 5. The data were obtained from traces of 53 nm x 16 nm GNRs in 95% glycerol. The triangles represent the theoretical values of ρ, obtained with Eq 7. In a), traces of all lengths were considered (between 10 and 100 points), while in b) only traces longer than 80 points (about 2 min) were considered. In both conditions there is not much difference using different numbers of fitting points. In c) we compare the values of ρ(D) obtained from traces of all lengths (from 10 to about 100 points, black squares) with the ones obtained from only traces longer than 40 points (blue squares) and 80 points (cyan); in all three cases the analysis was performed fixing the positional uncertainty