Fig. 6

Calcein AM translocate into GUVs and remained there also after it is removed from the bulk solution, but the fluorescent signal was relatively weak (I). Fluorescent signal representing calcein (a) or Alexa Fluor labeled dextran (b) remained intensive after the agents were removed from the bulk solution by repeated centrifugation. Inhibition of ATP hydrolysis caused a markedly increased formation of GPMVs, however the incidence of dextran positive vesicles (brighter vesicles) was low (c). GPMVs were imaged both in bright-field and epi-fluorescent mode and the images were then merged. The objective magnification was 60×. The white bar represents 10 μm