Copper-free click chemistry for attachment of biomolecules in magnetic tweezers
© Eeftens et al. 2015
Received: 24 April 2015
Accepted: 16 September 2015
Published: 25 September 2015
Single-molecule techniques have proven to be an excellent approach for quantitatively studying DNA-protein interactions at the single-molecule level. In magnetic tweezers, a force is applied to a biopolymer that is anchored between a glass surface and a magnetic bead. Whereas the relevant force regime for many biological processes is above 20pN, problems arise at these higher forces, since the molecule of interest can detach from the attachment points at the surface or the bead. Whereas many recipes for attachment of biopolymers have been developed, most methods do not suffice, as the molecules break at high force, or the attachment chemistry leads to nonspecific cross reactions with proteins.
Here, we demonstrate a novel attachment method using copper-free click chemistry, where a DBCO-tagged DNA molecule is bound to an azide-functionalized surface. We use this new technique to covalently attach DNA to a flow cell surface. We show that this technique results in covalently linked tethers that are torsionally constrained and withstand very high forces (>100pN) in magnetic tweezers.
This novel anchoring strategy using copper-free click chemistry allows to specifically and covalently link biomolecules, and conduct high-force single-molecule experiments. Excitingly, this advance opens up the possibility for single-molecule experiments on DNA-protein complexes and molecules that are taken directly from cell lysate.
Single-molecule methods have become increasingly popular to study biomolecules . With techniques such as atomic force spectroscopy, or optical or magnetic tweezers, one is able to study the mechanical properties of single DNA molecules, single proteins, or individual DNA-protein complexes. The effect of applied force on biomolecules is a particularly relevant topic, as mechanical forces play a crucial role in many cellular processes [2–4]. The relevant forces range from a few pN, like the force produced by an RNA polymerase during transcription (14pN) , to tens of pN, as in, for instance, viral packaging motors that use forces of 40pN to compact genomes . Even higher forces are needed in the process of chromosome segregation in eukaryotic cells, where microtubules pull on sister chromatids to segregate them to opposite sides of the spindle pole [7–10]. Many studies using magnetic tweezers have been published that probe the behavior of DNA-protein complexes under applied force and torque [11–16]. For studying biomolecules across the full relevant force range, it is necessary to also measure at higher forces (>20pN). In this regime, however, many traditional anchoring methods fail, thus limiting such single-molecule experiments.
Other, much stronger, anchoring methods have been developed [20–24] by functionalizing DNA with amine (Fig. 1b) or thiol groups (Fig. 1c) that are covalently linked to the surface or bead. Although these bonds indeed resist high forces, these techniques have an important limitation in that significant nonspecific binding occurs when studying systems that are more complicated than bare DNA. For example, when studying proteins, native lysines (amine) or cysteines (thiol) in the protein can bind nonspecifically (blue arrows in Fig. 1b, c). For controlled single-molecule measurements, it is however important that the force is being applied at a consistent and known location .
A new and exciting challenge is to study DNA-protein complexes that are extracted from cell lysate. For controlled single-molecule experiments, it is essential to anchor these complexes in a stable, strong, and specific way. As the anchoring methods developed so far are unsuitable, studying DNA-protein complexes or complexes from cell lysate remains challenging .
Here, we present a novel method for covalent attachment of a DNA tether to a surface, based on copper-free click chemistry. Click reactions are defined as those that are selective, with favorable reaction kinetics, a high yield, and good physiological stability. Early click chemistry reactions required copper as a catalyst . Copper is cytotoxic and thus limits application of click reactions in cells. More recently, copper-free methods became available, for instance the Strain Promoted Azide-Alkyne Click (SPAAC) reaction, of which the reaction between dibenzocyclooctyl (DBCO) and azide is an example . These click reactions are bio-orthogonal, i.e. they can occur within organisms without interfering with native biochemical processes.
A 20678 bp pSupercos1 plasmid was made by removal of the MluI fragment from pSupercos1 (Stratagene) and insertion of two lambda fragments. This Plasmid DNA was isolated with midiprep (Qiagen), restricted with XhoI and NotI.HF (New England Biolabs), and purified (Wizard® SV Gel and PCR Clean-Up System, Promega), resulting in a 20 kb fragment.
DBCO and biotin labeled handles were prepared by PCR on a pbluescriptIISK+ template (Stratagene) with a taq polymerase (GoTaq, Promega) and the addition of Biotin-16-dUTP (Roche), or 5-DBCO-dUTP (Jenabioscience) to the nucleotide mixture respectively. The forward primer was: GACCGAGATAGGGTTGAGTG, and reverse primer: CAGGGTCGGAACAGGAGAGC. The biotin-handle was digested with XhoI resulting in 554 bp and 684 bp fragments. The DBCO-handle was digested with NotI.HF resulting in 624 bp and 614 bp fragments. The handles were purified (Wizard® SV Gel and PCR Clean-Up System, Promega), combined with the restricted plasmid DNA and ligated with T4 DNA ligase (Promega) overnight at 16 °C. The tweezer-construct was then purified again (Wizard® SV Gel and PCR Clean-Up System, Promega).
Surface functionalization and flow cell assembly
For making amine-coated flow cells, coverslips (Menzel Glaser, 24x60mm, thickness #1) were cleaned in an O2 plasma cleaner for 30 s, which ensures activation of the silanol groups. Coverslips were then treated with 2 % APTES in acetone for 10 min, rinsed with MilliQ and air-dried. Before flow cell assembly, polystyrene beads (Polysciences Europe GmbH) were pipetted onto the coverslip and spread with the side of a pipette tip. These non-motile surface-bound beads serve as reference beads for drift correction. The amine-coated coverslips were then aligned with a pre-cut parafilm gasket and another coverslip (Fig. 2b). The assembled flow cell was put on a hot plate at 90 °C until the parafilm was sufficiently melted to prevent fluid leakage. The applied heat also firmly binds the polystyrene reference beads to the surface.
For control experiments, we used a dig-functionalized DNA construct. The dig handle was constructed in the same matter as the DBCO handle described above, but instead dig-11-dUTP was used (Digoxygenin-11-dUTP, Roche). Coverslips were cleaned in acetone for 30 min in a sonicator for creating the flow cells. After air-drying, they were coated with 1 % nitrocellulose (Invitrogen) in amylacetate (Sigma Aldrich). Application of reference beads and assembly of flow cells proceeded as described above. Next, nitrocellulose-coated flow cells were incubated with 100 mM anti-dig antibodies (Fab-fragment, Roche) for 30 min. After washing as described above, the surface was passivated with 10 mg/ml BSA (Bioke) for 1 h. Preparation of beads proceeded as described above. Beads with digoxygenin-functionalized DNA then incubated in the flow cell for 10 min. Finally, the flow cell was washed with washing buffer until no more unbound beads were visible.
Results and Discussion
We developed a protocol to covalently attach biomolecules in a magnetic tweezers flow cell using copper-free click chemistry. As described in Methods, we coat the glass surface with azide-functionalized PEG-linkers, and attach DBCO-tagged DNA through the azide-group, thereby covalently linking the DNA molecule at one end to the surface.
In contrast to the binding of DBCO to azide, the bond between biotin and streptavidin on the other end of the DNA is not covalent. Yet, as can be observed from Fig. 5, this bond also withstands forces of >100pN, which is consistent with earlier reports [17, 18]. For a wide range of applications, the current method, with tethers that contain a mutually orthogonal DBCO/azide bond on one end and biotin/streptavidin on the other, will suffice. Double copper-free click chemistry (with orthogonal click reactions at both bead and surface) can be considered in future applications if even much higher forces are desired.
The copper-free click chemistry attachment strategy presents many advantages. The reaction between DBCO and azide is relatively fast, specific, it does not require a catalyst, and, importantly for some applications, it can be performed in physiological conditions. Furthermore, azide and DBCO groups are relatively small and inert to biological moieties  and thus easy to incorporate. There are already numerous examples of the application of SPAAC reactions in biological systems and even living cells [28, 36]. Examples include use of copper-free click chemistry in non-canonical amino acids , imaging in live cells , joining of DNA strands , and DNA-functionalized nanoparticles .
Above, we demonstrated the use of a new DNA-attachment method in magnetic tweezers. We note that it can easily be applied to other single-molecule methods as well. For example, in the same manner, polystyrene beads could be coated with click chemistry functional groups for use in optical tweezers. By immobilizing the PEG linkers on the surface, the same copper-free click chemistry can also be used in atomic force microscopy , flow stretching and DNA combing.
Single-molecule force spectroscopy opens up the possibility to apply and measure forces on biomolecules, and study DNA-protein interactions. These in vitro experiments with bare DNA and purified protein give great insights into the cell machinery, but purified complexes are taken out of their cellular context. As our new method does not cross-react, it is possible to anchor and measure complexes that are directly extracted from cell lysate. Measuring on this native state of biomolecules can be expected to yield new insight into interactions between biomolecules.
Traditional methods for anchoring biomolecules have encountered limitations in studying DNA-protein complexes in magnetic tweezers related to low force stability and cross reactivity. Here, we developed a method for covalently anchoring biomolecules with copper-free click chemistry, using the reaction between DBCO and azide. This reaction is bio-orthogonal and no catalyst is needed. Furthermore, it is highly specific and it resists high force (>100pN). The protocol is reproducible, fast and uses commercially available reagents. Perhaps most excitingly, covalently linking molecules with copper-free click chemistry opens up the possibility to measure on a wide variety of DNA-protein complexes and complexes isolated from cell lysate.
We thank Jacob Kerssemakers for technical support and Richard Janissen for discussions. This work was supported by the ERC Advanced Grant NanoforBio (No. 247072) and by The Netherlands Organization for Scientific Research (NWO/OCW), as part of the Frontiers of Nanoscience program.
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